Biochemistry Lab

Biochemistry lab I. Introduction In this experiment, lipids from ground nutmeg ar extracted exploitation a crew of firmness of purposes and identify the lipids through chromatography. The use of goods and services of using firmness of purpose combinations is to elute the lipids establish on their mutual antonym to binding of the silica jelly. The chromatography is performed on a silica change plate and the use of iodin to visualize the lipids. By scheming the Rf values for for each one compound and comparing them to the eff lipids, we are able to signalize the lipids within the grounded nutmeg. II.

Procedures Add 5 g crushed nutmeg and 50 mL hexane-isopropanol into a flaskful and warm for 15 minutes. Remove the extra solvent on a steam clean bath under a hood while flushing the flask with N2 gas, leaving the crude extract. consult extract. Prepare silica gelatine tower. Add 6 g of silica gel in 20 mL of hexane to scan a slurry. Block column with small piece of deoxyephedrine wool, add 5 mL of hexane and then add the silica slurry up t...If you want to dispirit a full essay, submit it on our website: Ordercustompaper.com

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